重庆快乐十分

Genetic diversity of Bartonella quintana in macaques suggests zoonotic origin of trench fever

基因多样性

Genetic diversity of Bartonella quintana in macaques suggests zoonotic origin of trench fever

HAO LI,*1JIE-YING BAI,†1LI-YUAN WANG,*‡LIN ZENG,†YAN-SH ENG SHI,†

ZHENG-LIANG QIU,†HUA-HU YE,†XIAO-FEI ZHANG,†QING-BIN LU,*§MICHAEL KOSOY,¶WEI LIU*and WU-CHUN C AO*

*State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Beijing,People’s Republic of China,†Laboratory Animal Center,Academy of Military Medical Sciences,Beijing,People’s Republic of China,‡Graduate School of Anhui Medical University,Hefei,People’s Republic of China,§School of Public Health,Shangdong

University,Jinan,People’s Republic of China,¶Division of Vector Borne Diseases,Centers for Disease Control and Prevention,Fort Collins,Colorado,USA

Abstract

Bartonella quintana is a bacterium that causes a broad spectrum of diseases in humans

including trench fever.Humans were previously considered to be the primary,if not

the only,reservoir hosts for B。quintana 。To identify the animal reservoir and extend

our understanding of the ecological and evolutionary history of B.quintana ,we exam-

ined blood samples from macaques and performed multilocus sequence typing

重庆快乐十分(MLST)analysis。We demonstrated the prevalence of B。quintana infection was com-

mon in macaques from main primate centres in mainland China.Overall,18.0%(59/

328)of rhesus macaques and 12.7%(39/308)of cynomolgus macaques were found to be

infected with B.quintana by blood culture and/or polymerase chain reaction.The

infection was more frequently identified in juvenile and young monkeys compared

with adult animals.In contrast with the relatively low level of sequence divergence of

B。quintana reported in humans,our investigation revealed much higher genetic diver-

sity in nonhuman primates.We identified 44new nucleotide variable sites and 14

novel sequence types (STs)among the B.quintana isolates by MLST analysis.Some

STs were found only in cynomolgus macaques,while some others were detected only

in rhesus macaques,suggesting evidence of host-cospeciation,which were further

confirmed by phylogenetic analysis and Splits decomposition analysis.Our findings

suggest that trench fever may primarily be a zoonotic disease with macaques as the

natural hosts。

Keywords :Bacteria,Bartonella quintana ,macaque,MLST,trench fever,zoonotic disease

Received 9December 2012;revision received 13January 2013;accepted 15January 2013

重庆快乐十分Introduction

Bartonella quintana is a fastidious gram-negative bacte-

rium that was originally recognized as the causative

agent for trench fever during World War I.The spec-

trum of human diseases associated with this organism

now includes chronic bacteremia,endocarditis and bacillary angiomatosis (Drancourt et al.1995;Koehler et al.1997;Brouqui et al.1999;Klein et al.2002).For a long time,humans were considered to be the only reser-voir host for B.quintana ,which contrasts with most other Bartonella species that have identified animal reser-voirs.Although B.quintana DNA has been detected in cat fleas,the dental pulp of a cat (Rolain et al.2003;La et al.2005;Breitschwerdt et al.2007),as well as in two endocarditic dogs (Kelly et al.2006),no reliable evidence is available to support the suggestions that cats and/or dogs serve as competent reservoirs for B.quintana .Correspondence:Wu-Chun Cao,Fax:+86-10-63896082;

E-mail:caowc@http://www.like2us.com/doc/info-df712954ad51f01dc281f1a0.html ;Wei Liu,Fax:+86-10-63896082;

E-mail:liu_weis@http://www.like2us.com/doc/info-df712954ad51f01dc281f1a0.html ;Michael Kosoy,

Fax:(970)225-4257;E-mail:mck3@cdc。gov

1Hao Li and Jieying Bai contributed equally to this work.

©2013Blackwell Publishing Ltd Molecular Ecology (2013)doi:

Genetic diversity of Bartonella quintana in macaques suggests zoonotic origin of trench fever

10.1111/mec.12261

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